#41014 Cellular and molecular basis of neuroinflammation: potential targets for translational medicine and therapy

The research focus of this project is to highlight some cellular and molecular mechanisms which underlie neuroinflammation, to identify potential target molecules and signaling pathways. Our aim is to find adequate therapeutic approaches for sanitation of neuroinflammation and promotion of regeneration after the central nervous system (CNS) damage. The efficacy of several therapeutic approaches (purine nucleoside analogues: Ribavirin and Tiazofurin, hyperbaric oxygenation, B vitamins - B1, B2, B3, B6, B12) in suppression of detrimental and enhancement of beneficial inflammatory mechanisms is tested. Considering the pivotal role of extracellular purine nucleotides and nucleosides in the control of neurodegenerative processes and inflammatory responses a particular focus will be put on regulation of their metabolism and the role of ectonucleotidases.

In different animal models (brain injury, multiple sclerosis: experimental autoimmune encephalomyelitis, cuprizone-induced demyelination) behavioral, cellular and molecular approaches (enzymatic assays, microdialysis combined with HPLC and bioluminescent detection, RT-PCR, immunoblot and immunohistochemistry, ELISA, confocal microscopy and whole cell patch clamp) are used. Besides in vivo neuroinflammatory processes are also studied in vitro in primary astrocytic and microglial cell culture, and glial cell lines. The main goal of the project is to identify, characterize and standardize potential early and sensitive biomarkers of neuroinflammation and neurodegeneration that can be used in clinical and laboratory practice for diagnosis and prognosis of patient outcome after damage to the CNS and for assessing the effectiveness of applied therapies. The project consists of 2 subprojects:

The role of purinergic signalling in acute and chronic neuroinflammation (principal investigator, prof. Nadežda Nedeljković).
Investigation of potential therapeutic approaches for sanitation of neuroinflammation and promotion of regeneration after damage to the central nervous system (principal investigator, Sanja Peković, PhD).

Project team:

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Dr. Sanja Peković

Dr. Irena Lavrnja

Dr. Ivana Bjelobaba

Dr. Danijela Savić

Dr. Nataša Todorović

Dr. Ana Parabucki

Dr. Iva Božić

Marija Jovanović

Other Collaborators:

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Dr. Thomas Herdegen

Dr. Markus Kipp

Dr. Radmila Filipović

Faculty of Biology, University of Belgrade:

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School of Medicine, University of Belgrade:

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Vinča Institute of Nuclear Sciences, University of Belgrade:

Dr. Ivana Stojanović

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Address

Bulevar despota Stefana 142
11108 Belgrade
Serbia

Contact

Telefon: +381 11 20 78 300
Fax: +381 11 27 61 433
Email: ibiss@ibiss.bg.ac.rs

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