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Institute for Biological Research "Siniša Stanković"
National Institute of the Republic of Serbia
University of Belgrade

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Pubertal Triptorelin Exposure Alters Hypothalamic Reproductive–Metabolic Integration in a Sex-Specific Manner

Gonadotropin-releasing hormone agonists are used to treat central precocious puberty and are increasingly prescribed in gender-affirming care. Despite well-established reproductive effects, their broader physiological effects during puberty remain poorly understood. Male and female Wistar rats received a depot triptorelin injection at pubertal onset. Body mass, food intake, and locomotor activity were monitored. After 28 days, reproductive function was assessed, and pituitary and hypothalamic gene...

Understanding the Role of Hypothalamic–Pituitary–Gonadal Axis in Multiple Sclerosis and Experimental Autoimmune...

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system, mostly occurring during the reproductive period, and it is diagnosed more frequently in women. The sex biases in the incidence and course of both MS and its most commonly used animal model, experimental autoimmune encephalomyelitis (EAE), seem to be driven by fluctuations in sex hormones. The regulation of sex hormone synthesis and release relies on a dynamic hierarchical system...

Tetramic acid-derived blue-emitting fluorescent probe for selective lipid droplet imaging in live cells and zebrafish

Lipid droplets (LDs) are dynamic intracellular organelles involved in lipid storage and metabolism, and their imaging is essential for studying metabolic processes in health and disease. Here, we describe four novel fluorescent tetramic acid derivatives as potential LD markers for live imaging in cellular and whole-organism systems, and designate the compound with the most favorable properties, 1a, for further characterization. The probe 1a was evaluated in multiple cell lines, where it enabled clear...

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