Selected publications - The effects of magnetic fields and other environmental stressors on the physiological responses and behavior of different species

1. Spasić S, Kesić S, Stojadinović G, Peković B, Todorović D (2015): Effects of the static and ELF magnetic fields on the neuronal population activity in Morimus funereus (Coleoptera, Cerambycidae) antennal lobe revealed by wavelet analysis. Comp Biochem Physiol A Mol Integr Physiol 181:27-35.
2. Vlahović M, Ilijin L, Lazarević J, Mrdaković M, Gavrilović A, Matić D, Perić-Mataruga V (2014): Cadmium-induced changes of gypsy moth larval mass and protease activity. Comp Biochem Physiol C Toxicol Pharmacol 160:9-14.
3. Nikolić L, Bataveljić D, Andjus PR, Moldovan I, Nedeljković M, Petković B (2014): Modification of glial response in hibernation: a patch-clamp study on glial cells acutely isolated from hibernating land snail. J Biol Rhythms 29(6):442-455.
4. Ilijin L, Vlahović M, Mrdaković M, Mirčić D, Todorović D, Lazarević J, Perić-Mataruga V (2014): Responses of PTTH-producing neurosecretory neurons in Lymantria dispar caterpillars exposed to cadmium. Environ Toxicol 29(7):770-779.
5. Perić-Mataruga V, Vlahović M, Mrdaković M, Todorović D, Matić D, Gavrilović A, Ilijin L (2014): Prothoracicotropic hormone-producing neurosecretory neurons and antioxidative defense in midgut of Lymantria dispar in trophic stress. Tur J Biol 38(3):403-411.
6. Kesić S, Nikolić Lj, Savić A, Janać B, Spasić S (2014): Ouabain modulation of snail Br neuron bursting activity after the exposure to 10 mT static magnetic field revealed by Higuchi fractal dimension. Gen Physiol Biophys 33(3):335-344.
7. Mrdaković M, Stojković B, Perić-Mataruga V, Ilijin L, Vlahović M, Lazarević J (2014): Adaptive phenotypic plasticity of gypsy moth digestive enzymes. Cent Eur J Biol 9(3):309-319.
8. Todorović D, Marković T, Prolić Z, Mihajlović S, Rauš S, Nikolić L, Janać B (2013): The influence of static magnetic field (50 mT) on development and motor behaviour of Tenebrio (Insecta, Coleoptera). Int J Radiat Biol 89(1):44-50.
9. Vlahović M, Perić Mataruga V, Mrdaković M, Matić D, Lazarević J, Nenadović V, Ilijin L (2013): Enzymatic biomarkers as indicators of dietary cadmium in gypsy moth caterpillars. Environ Sci Pollut Res 20(5):3447-3455.
10. Selaković V, Rauš Balind S, Radenović L, Prolić Z, Janać B (2013): Age-dependent effects of ELF-MF on oxidative stress in the brain of Mongolian gerbils. Cell Biochem Biophys 66(3):513-521.
11. Nikolić L, Bataveljić D, Andjus PR, Nedeljković M, Todorović D, Janać B (2013): Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field. J Exp Biol 216(18):3531-3541.
12. Mirčić D, Blagojević D, Perić-Mataruga V, Ilijin L, Mrdaković M, Vlahović M, Lazarević J (2013): Cadmium effects on the fitness-related traits and antioxidative defense of Lymantria dispar L. larvae. Environ Sci Pollut Res 20(1):209-218.
13. Rauš S, Selaković V, Manojlović-Stojanoski M, Radenović L, Prolić Z, Janać B (2013): Response of hippocampal neurons and glial cells to alternating magnetic field in gerbils submitted to global cerebral ischemia. Neurotox Res 23(1):79-91.
14. Mrdaković M, Perić-Mataruga V, Ilijin L, Vlahović M, Janković-Tomanić M, Mirčić D, Lazarević J (2013): Response of Lymantria dispar (Lepidoptera: Lymantriidae) larvae from differently adapted populations to allelochemical stress: Effects of tannic acid. Eur J Entomol 110(1):55-63.
15. Janać B, Selaković V, Rauš S, Radenović L, Zrnić M, Prolić Z (2012): Temporal patterns of extremely low frequency magnetic field-induced motor behavior changes in Mongolian gerbils of different age. Int J Radiat Biol 88(4):359-366.
16. Todorović D, Mirčić D, Ilijin L, Mrdaković M, Vlahović M, Prolić Z, Perić-Mataruga V (2012): Effect of magnetic fields on antioxidative defense and fitness-related traits of Baculum extradentatum (Insecta, Phasmatodea). Bioelectromagnetics 33(3):265-273.
17. Rauš S, Selaković V, Radenović L, Prolić Z, Janać B (2012): Extremely low frequency magnetic field induced changes in motor behaviour of gerbils submitted to global cerebral ischemia. Behav Brain Res 228(2):241-246.
18. Perić-Mataruga V, Vlahović M, Janać B, Ilijin L, Janković-Tomanić M, Matić D, Mrdaković M (2012): Ghrelin effect on nutritional indices, midgut and fat body of Lymantria dispar L. (Lymantriidae). Peptides 37(1)׃55-62.
19. Janković-Tomanić M, Lazarević J (2012): Effects of temperature and dietary nitrogen on genetic variation and covariation in gypsy moth larval performance traits. Arch Biol Sci, Belgrade 64(3):1109-1116.
20. Ilijin L, Vlahović M, Mrdaković M, Mirčić D, Prolić Z, Lazarević J, Perić-Mataruga V (2011): The effects of acute exposure to magnetic fields on morphometric characteristics of bombyxin-producing neurosecretory neurons in gypsy moth caterpillars. Int J Radiat Biol 87(5):461-471.

Selected publications- The role of autophagy in the regulation of tumor cell death

1. Zogovic N, Tovilovic-Kovacevic G, Misirkic-Marjanovic M, Vucicevic L, Janjetovic K, Harhaji-Trajkovic L, Trajkovic V. Coordinated activation of AMP-activated protein kinase, extracellular signal-regulated kinase, and autophagy regulates phorbol myristate acetate-induced differentiation of SH-SY5Y neuroblastoma cells. J Neurochem. 2015;133(2):223–32.
2. Ristic B, Bosnjak M, Arsikin K, Mircic A, Suzin-Zivkovic V, Bogdanovic A, Perovic V, Martinovic T, Kravic-Stevovic T, Bumbasirevic V, Trajkovic V, Harhaji-Trajkovic L. Idarubicin induces mTOR-dependent cytotoxic autophagy in leukemic cells. Exp Cell Res. 2014;326(1):90-102.
3. Bosnjak M, Ristic B, Arsikin K, Mircic A, Suzin-Zivkovic V, Perovic V, Bogdanovic A, Paunovic V, Markovic I, Bumbasirevic V, Trajkovic V, Harhaji-Trajkovic L. Inhibition of mTOR-dependent autophagy sensitizes leukemic cells to cytarabine-induced apoptotic death. PLoS One. 2014;9(4):e94374.
4. Tovilovic G, Zogovic N, Soskic V, Schrattenholz A, Kostic-Rajacic S, Misirkic-Marjanovic M, Janjetovic K, Vucicevic L, Arsikin K, Harhaji-Trajkovic L, Trajkovic V. Arylpiperazine-mediated activation of Akt protects SH-SY5Y neuroblastoma cells from 6-hydroxydopamine-induced apoptotic and autophagic death. Neuropharmacology. 2013;72:224-35.
5. Pantovic A, Krstic A, Janjetovic K, Kocic J, Harhaji-Trajkovic L, Bugarski D, Trajkovic V. Coordinated time-dependent modulation of AMPK/Akt/mTOR signaling and autophagy controls osteogenic differentiation of human mesenchymal stem cells. Bone. 2013;52(1):524-31.
6. Harhaji-Trajkovic L, Arsikin K, Kravic-Stevovic T, Petricevic S, Tovilovic G, Pantovic A, Zogovic N, Ristic B, Janjetovic K, Bumbasirevic V, Trajkovic V. Chloroquine-mediated lysosomal dysfunction enhances the anticancer effect of nutrient deprivation. Pharm Res. 2012;29(8):2249-63.
7. Tovilovic G, Zogovic N, Harhaji-Trajkovic L, Misirkic-Marjanovic M, Janjetovic K, Vucicevic L, Kostic-Rajacic S, Schrattenholz A, Isakovic A, Soskic V, Trajkovic V. Arylpiperazine dopamineric ligands protect neuroblastoma cells from nitric oxide (NO)-induced mitochondrial damage and apoptosis. ChemMedChem. 2012;7(3):495-508.
8. Janjetovic K, Harhaji-Trajkovic L, Misirkic-Marjanovic M, Vucicevic L, Stevanovic D, Zogovic N, Sumarac-Dumanovic M, Micic D, Trajkovic V. In vitro and in vivo anti-melanoma action of metformin. Eur J harmacol. 2011;668(3):373-82.
9. Janjetovic K, Vucicevic L, Misirkic M, Vilimanovich U, Tovilovic G, Zogovic N, Nikolic Z, Jovanovic S, Bumbasirevic V, Trajkovic V, Harhaji-Trajkovic L.. Metformin reduces cisplatin-mediated apoptotic death of cancer cells through AMPK-independent activation of Akt. Eur J Pharmacol. 2011;651(1-3):41-50.
10. Vucicevic L, Misirkic M, Janjetovic K, Vilimanovich U, Sudar E, Isenovic E, Prica M, Harhaji-Trajkovic L, Kravic-Stevovic T, Bumbasirevic V, Trajkovic V. Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway. Autophagy. 2011;7(1):40-50.
11. Vucicevic L, Misirkic-Marjanovic M, Paunovic V, Kravic-Stevovic T, Martinovic T, Ciric D, Maric N, Petricevic S, Harhaji-Trajkovic L, Bumbasirevic V, Trajkovic V. Autophagy inhibition uncovers the neurotoxic action of the antipsychotic drug olanzapine. Autophagy. 2014;10(12):2362-78.
12. Arsikin K, Kravic-Stevovic T, Jovanovic M, Ristic B, Tovilovic G, Zogovic N, Bumbasirevic V, Trajkovic V, Harhaji-Trajkovic L. Autophagy-dependent and independent involvement of AMP-activated protein kinase in 6-hydroxydopamine toxicity to SH-SY5Y neuroblastoma cells. Biochim Biophys Acta. 2012;1822(11):1826-36.
13. Pantic I, Harhaji-Trajkovic L, Pantovic A, Milosevic NT, Trajkovic V. Changes in fractal dimension and lacunarity as early markers of UV-induced apoptosis. J Theor Biol. 2012;303:87-92.

Selected publications - Neurological development in prenatal exposure to antiepileptic drugs

1. Martać Lj, Podgorac J, Sekulić S. Evaluation of the neurotoxical effect of aluminium on the wistar rat. Arch Biol Sci. 2010;62:585-588.
2. Sekulić SR, Ilić Đ, Novakov-Mikić A. Polyhydramnios and bone development: an unexplored relationship. Med Hypothes. 2010;75:312-4.
3. Sekulić SR, Mikov A, Petrović Đ. Probability for breech presentation and its significance. J Mater Fet Neonat Med. 2010;23:1160-4.
4. Keković G, Ćulić M, Martać M, Stojadinović G, Čapo I, Lalošević D, Sekulić S. Fractal dimension values of cerebral and cerebellar activity in rats loaded with aluminium. Med Biol Eng Comput. 2010;48:671-9.
5. Martać Lj, Grbić G, Keković G, Podgorac J, Ćulić M, Sekulić S, Lalošević D, Čapo I. Spectral changes of brain activity in offsping of rats exposed to aluminium during gestation and lactation. Arch Biol Sci. 2010;62:9-13.
6. Sekulić S, Naumović N, Lukač D, Stefanović S, Mikov A, Sakač D, Kopitović A. Ultrasound assessment of the effect of fetal position on the supine to prone righting reflex in guinea pig fetus. Period Biol. 2010;112:97-104.
7. Keković G, Stojadinović G, Martać Lj, Podgorac J, Sekulić S, Culić M. Spectral and Fractal Measures of Cerebellar and Cerebral Activity in Various Types of Anaesthesia. Acta Neurobiol Exp. 2010;70:67-75.
8. Sekulić S, Lukač D, Drapšin M, SuknjajaV, Keković G, Grbić G, Martać Lj. The righting reflex from a supine to a prone position in the guinea pig fetus. Gen Physiol Biophys. 2009;28:284-8.
9. Ćulić M, Keković G, Grbić G, Martać Lj, Soković K, Podgorac J, Sekulić S. Wavelet and Fractal Analysis of Rat Brain Activity in Seizures Evoked by Camphor Essential Oil and 1, 8-Cineole. Gen Physiol Biophis. 2009;28:33-40.
10. Sekulić SR, Žarkov M, Slankamenac P, Božić K, Vejnović T, Novakov-Mikić A. Decreased Expression of the Righting Reflex and Locomotor Movements in Breech-Presenting Newborns in the First Days of Life. Early Hum Develop. 2009;85:263-6.
11. Sekulić SR, Lukač D, Drapšin M, Čapo I, Lalošević D, Novakov-Mikić A. Ultrasonographic observation of maturation of basic movements in guinea pig fetuses. Cent Eur J Biol. 2009;4:58-61.
12. Spasić S, Ćulić M, Grbić G, Martać Lj, Sekulić S, Mutavdžić D. Spectral and fractal analysis of cerebellar activity after single and repeated brain njury. Bull Math Biol. 2008;70:1235-49.
13. Sekulic SR, Lalosevic D, Naumovic N, Putic S, Culic M. The probability for anterior and posterior presentation during the second half of gestation and at delivery among albino mice. Biologia (Bratislava). 2007;62:622-5.
14. Sekulic SR, Petrovic Đ, Runic R, Williams M, Vejnovic T. Does more than 50% of breech presentation exist? Twin Res Hum Genet 2007; 10: 649-54.
15. Sekulic SR, Bozic K, Bozic A, Borota J, Milka C. Precocial rodents as a new experimental model to study the effects of altered gravitational condition on fetal development. Micro Sci Tech. 2006;18:223-5.
16. Grbić GM, Ćulić M, Martać Lj, Soković MD, Spasić SZ, Djoković DD Effect Of Camphor Essential Oil On Rat Cerebral Cortex Activity As Manifested By Fractal Dimension Changes. Arch Biol Sci. 2008;60:547-53.
17. Culić M, Stojadinović G, Martać Lj, Soković MD . Use of the Hurst Exponent for Analysis of Electrocortical Epileptiform Activity Induced in Rats by Administration of Camphor Essential Oil or 1,8-Cineole. Neurophysiology. 2010;42:57-62.

During the development of new research trends at the Institute, the Department of Cryophysiology under the supervision of Academician Prof. Dr. Radoslav Anđus was established in the early 1960s. Later, the Department of Neurophysiology was established thanks to Dr. Mira Pašić, professor of the Faculty of Biology. The main objective was to examine the neurophysiological basis of behavior at the cellular level.

Using classical electrophysiological techniques (current clamp and voltage clamp), staff of the Department under the supervision of Dr. Gordana Kartelija contributed to establishing the fundamental biophysical properties of individual neurons of the snail Helix pomatia. This research was a starting point for further analysis of functional properties of neurons affected by different active substances and environmental factors (temperature, light, magnetic fields,...) and the mechanisms of neuromodulation.

In addition to classical electrophysiological studies on the snail ganglion complex, recently the biophysical properties of vertebrate nerve cells are analyzed using modern techniques. The patch clamp technique is used to examine the whole-cell ionic currents and the properties of single ion channels in cultured nerve cells.

Electrophysiological signals are analyzed using linear and nonlinear mathematical methods (wavelet and fractal analysis), and advanced statistical methods (independent component analysis). Application of these methods allows detailed analysis of the activity of individual neurons and neuronal populations.

Scientific interest of the Department is extended towards investigation of morpho-functional characteristics of the brain and behavior of experimental animals. The effects of different neuroactive substances, magnetic fields and aging are estimated in physiological and pathophysiological conditions. Neuronal and glial cells are analyzed using immunohistochemical labeling methods and changes of some biochemical parameters in the brain are examined. Animal behavior in specific tests (open field, plus maze, Porsolt forced swim, object recognition, light/dark box,...) is automatically monitored and analyzed using the Any-maze software.

Emphasis is placed on clearly defined neurological and psychiatric diseases in humans. The pathogenic mechanisms involved in the onset of these disorders and pharmacologically active substances which could act protective are studied using animal models (chemically induced seizures for epilepsy and application of aluminum that is associated with the etiology of Alzheimer's disease). These aims are examined on the basis of biochemical and immunohistochemical changes in some brain structures and clinical manifestation of disorders.

GENERAL TOPICS:

Electrophysiology of nerve cells
Analysis of electrophysiological signals
Morpho-functional characteristics of the brain
Animal behavior
Neuroactive substances
Magnetic fields
Animal models

Department of Neurobiology is founded in the early sixties, by Acad. Prof. Ljubisav Rakić at the "Siniša Stanković" Institute for Biological Research, University of Belgrade. During the last 50 years, the investigations at the Department have contributed to the examination of fundamental nervous system processes. Department of Neurobiology consists of the following laboratories: Molecular Neurobiology, Neurochemistry and Neurophysiology, and represents the scientific ground for undergraduate and PhD programs. Presently, there are 40 members of Department. Members of the Department are lecturers at undergraduate and PhD studies (programme: Molecular Biology and Neurosciences) at the Faculty of Biology (BF), University of Belgrade.

The research at the Department is focused on several areas:

Behavioral and electrophysiological studies of the CNS
The biochemical organization of the CNS
Neuroimmunology
Molecular and behavioral studies of brain plasticity
Neurodegenerative diseases of the CNS
Cancer and gene therapy of brain tumors (animal model of multi-drug resistant glioma, mechanisms of multi-drug resistance, new therapeutic approaches)
Biochemical and molecular basis of brain injury and repair
Therapeutical and pharmaceutical approaches in the treatment of CNS disorders
The neurobiology of sleep and sleep-related breathing in the animal models of healthy aging and neurodegenerative diseases, and identification of the neural networks responsible for the regulation of sleep and breathing pattern in different sleep/wake states

Neurobiology of sleep in aging and disease - electroencephalographic markers and modeling in the estimation of disorder #173022

1. Saponjic J, Cvorovic J, Radulovacki M, Carley DW. Serotonin and noradrenaline modulate respiratory pattern disturbance evoked by glutamate injection into the pedunculopontine tegmentum of anesthetized rats. Sleep. 2005;28:560-70.
2. Saponjic J, Radulovacki M, Carley DW. Modulation of respiratory pattern and upper airway muscle activity by the pedunculopontine tegmentum: Role of NMDA receptors. Sleep Breath. 2006;10:195-202.
3. Saponjic J, Radulovacki M, Carley DW. Monoaminergic system lesions increase post-sigh respiratory pattern disturbance during sleep in rats. Physiol Behav. 2007;90:1-10.
4. Bojic T, SaponjicJ, Radulovacki M, Carley DW, Kalauzi A. Monotone signal segments analysis as a novel method of breath detection and breath-to-breath interval analysis in rat. Respir Physiol Neurobiol. 2008;161:273-80.
5. Bjelobaba I, Saponjic J. High volume microinfusion suppresses local astrocyte response within nucleus basalis of rat. Arch Ital Biol. 2013;151:24-32.
6. Saponjic J, Petrovic J, Kalauzi A, Ciric J, Lazic K, Radulovacki M, Carley DW. Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder. Sleep Biol Rhythms. 2013;11(2):105-15.
7. Petrovic J, Ciric J, Lazic K, Kalauzi A, Saponjic J. Lesion of the pedunculopontine tegmental nucleus in rat augments cortical activation and disturbs sleep/wake state transitions structure. Exp Neurol. 2013;247:562-71.
8. Petrovic J, Lazic K, Ciric J, Kalauzi A, Saponjic J. Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat. Behav Brain Res. 2013;256:108-18.
9. Petrovic J, Lazic K, Kalauzi A, Saponjic J. REM sleep diversity following the pedunculopontine tegmental nucleus lesion in rat. Behav Brain Res. 2014;271:258–68.
10. Bataveljic D, Petrovic J, Lazic K, Saponjic J, Andjus P. Glial response in the rat models of functionally distinct cholinergic neuronal denervations. J Neurosci Res. 2015;93(2):244-52.
11. Ciric J, Lazic K, Petrovic J, Kalauzi A, Saponjic J. Aging induced cortical drive alterations during sleep in rats. Mech Ageing Dev. 2015;146-148C:12–22.
12. Lazic K, Petrovic J, Ciric J, Kalauzi a., Saponjic J. Impact of anesthetic regimen on the respiratory pattern, EEG microstructure and sleep in the rat model of cholinergic Parkinson’s disease neuropathology. Neuroscience. 2015;304:1–13.

Brain plasticity in aging: effect of dietary restriction and anesthesia #173056

1. Tesic V, Perovic M, Lazic D, Kojic S, Smiljanic K, Ruzdijic S, Rakic L, Kanazir S. Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat. J Steroid Biochem Mol Biol. 2015;149C:43-52.
2. Smiljanic K, Pesic V, Mladenovic Djordjevic A, Pavkovic Z, Brkic M, Ruzdijic S, Kanazir S. Long-term dietary restriction differentially affects the expression of BDNF and its receptors in the cortex and hippocampus of middle-aged and aged male rats. Biogerontology. 2015;16:71-83.
3. Smiljanic K, Vanmierlo T, Mladenovic-Djordjevic A, Perovic M, Ivkovic S, Luetjohann D, Kanazir S. Cholesterol metabolism changes under long-term dietary restrictions while the cholesterol homeostasis remains unaffected in the cortex and hippocampus of aging rats. AGE. 2014;36(3):1303-14.
4. Milanović D, Pešić V, Popić J, Tanić N, Kanazir S, Jevtović-Todorović V, Ruždijić S. Propofol anesthesia induces proapoptotic tumor necrosis factor-α and pro-nerve growth factor signaling and prosurvival Akt and XIAP expression in neonatal rat brain. J Neurosci Res. 2014;92(10):1362-73.
5. Mladenovic Djordjevic A, Perovic M, Smiljanic K, Todorovic S, Tesic V, Ruzdijic S, Kanazir S. The effects of dietary restriction and aging on amyloid precursor protein and presenilin-1 mRNA and protein expression in rat brain. Neuroreport. 2014;25(6):398-403.
6. Perovic M, Tesic V, Mladenovic Djordjevic A, Smiljanic K, Loncarevic-Vasiljkovic N, Ruzdijic S, Kanazir S. BDNF transcripts, proBDNF and proNGF, in the cortex and hippocampus throughout the life span of the rat. AGE. 2013;35(6):2057-70.
7. Smiljanic K, Vanmierlo T, Mladenovic Djordjevic A, Perovic M, Loncarevic-Vasiljkovic N, Tesic V, Rakic Lj, Ruzdijic S, Lutjohann D, Kanazir S. Aging induces tissue-specific changes in cholesterol metabolism in rat brain and liver. Lipids. 2013;48(11):1069-77.
8. Popic J, Pesic V, Milanovic D, Todorovic S, Kanazir S, Jevtovic-Todorovic V, Ruzdijic S. Propofol-induced changes in neurotrophic signaling in the developing nervous system in vivo. PLoS One. 2012;7(4):e34396.
9. Loncarevic-Vasiljkovic N, Pesic V, Todorovic S, Popic J, Smiljanic K, Milanovic D, Ruzdijic S, Kanazir S. Caloric restriction suppresses microglial activation and prevents neuroapoptosis following cortical injury in rats. PLoS One. 2012;7(5):e37215.
10. Milanovic D, Popic J, Pesic V, Loncarevic-Vasiljkovic N, Kanazir S, Jevtovic-Todorovic V, Ruzdijic S. Regional and temporal profiles of calpain and caspase-3 activities in postnatal rat brain following repeated propofol administration. Dev Neurosci. 2010;32(4):288-301.
11. Pesić V, Milanović D, Tanić N, Popić J, Kanazir S, Jevtović-Todorović V, Ruzdijić S. Potential mechanism of cell death in the developing rat brain induced by propofol anesthesia. Int J Dev Neurosci. 2009;27(3):279-87

Cellular and molecular basis of neuroinflammation: potential targets for translational medicine and therapy #41014

1. Božić I, Savić D, Laketa D, Bjelobaba I, Milenković I, Peković S, Nedeljković N, Lavrnja I. Benfotiamine Attenuates Inflammatory Response in LPS Stimulated BV-2 Microglia. PLoS One. 2015;10(2):e0118372.
2. Lavrnja I, Trifunovic S, Ajdzanovic V, Pekovic S, Bjelobaba I, Stojiljkovic M, Milosevic V. Sensorimotor cortex ablation induces time-dependent response of ACTH cells in adult rats: behavioral, immunohistomorphometric and hormonal study. Physiol Behav. 2014;125:30–7.
3. Lavrnja I, Ajdzanovic V, Trifunovic S, Savic D, Milosevic V, Stojiljkovic M, Pekovic S. Cortical ablation induces time-dependent changes in rat pituitary somatotrophs and upregulates growth hormone receptor expression in the injured cortex. J Neurosci Res. 2014;92(10):1338–49.
4. Lavrnja I, Laketa D, Savic D, Bozic I, Bjelobaba I, Pekovic S, Nedeljkovic N. Expression of a second ecto-5'-nucleotidase variant besides the usual protein in symptomatic phase of experimental autoimmune encephalomyelitis. J Mol Neurosci. 2015;55(4):898–911.
5. Savić D, Stojiljkovic M, Lavrnja I, Parabucki A, Bjelobaba I, Nedeljkovic N, Herdegen T, Pekovic S. Ribavirin shows immunomodulatory effects on activated microglia. Immunopharmacol Immunotoxicol. 2014;36(6):433–41.
6. Brkic P, Stojiljkovic M, Jovanovic T, Dacic S, Lavrnja I, Savic D, Parabucki A, Bjelobaba I, Rakic L, Pekovic S. Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats. Brain Injury. 2012. p. 1273–84.
7. Parabucki AB, Bozić ID, Bjelobaba IM, Lavrnja IC, Brkić PD, Jovanović TS, Savić DZ, Stojiljković MB, Peković SM. Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats. Croat Med J. 2012;53(6):586–97.
8. Lavrnja I, Savic D, Bjelobaba I, Dacic S, Bozic I, Parabucki A, Nedeljkovic N, Pekovic S, Rakic L, Stojiljkovic M. The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis. Journal of Pharmacological Sciences. 2012. p. 221–32.
9. Bjelobaba I, Lavrnja I, Parabucki A, Stojkov D, Stojiljkovic M, Pekovic S, Nedeljkovic N. The cortical stab injury induces beading of fibers expressing ecto-nucleoside triphosphate diphosphohydrolase 3. Neuroscience. 2010;170:107-16.
10. Laketa D, Bjelobaba I, Savic J, Lavrnja I, Stojiljkovic M, Rakic L, Nedeljkovic N. Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum. Mol Cell Biochem. 2010;339:99-106.
11. Lavrnja I, Stojkov D, Bjelobaba I, Pekovic S, Dacic S, Nedeljkovic N, Mostarica-Stojkovic M, Stosic-Grujicic S, Rakic L, Stojiljkovic M. Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production. Int Immunopharmacol. 2008;8:1282-90.
12. Stojkov D, Lavrnja I, Pekovic S, Dacic S, Bjelobaba I, Mostarica-Stojkovic M, Stosic-Grujicic S, Jovanovic S, Nedeljkovic N, Rakic L, Stojiljkovic M. Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: clinical and histopathological evaluation. J Neurol Sci. 2008;267:76-85
13. Nedeljkovic N, Bjelobaba I, Subasic S, Lavrnja I, Pekovic S, Stojkov D, Vjestica A, Rakic L, Stojiljkovic M. Up-regulation of ectonucleotidase activity after cortical stab injury in rats. Cell Biol Int. 2006;306:541-6.
14. Pekovic S, Subasic S, Nedeljkovic N, Bjelobaba I, Fillipovic R, Milenkovic I, Lavrnja I, Stojkov D, Jovanovic S, Rakic Lj, Stojiljkovic M. Molecular basis of brain injury and repair. In: Ruzdijic S, Rakic Lj, editors. Neurobiological studies – From genes to behavior. Keral: Research Signpost; 2006.p.143-65.
15. Peković S, Filipović R, Subasić S, Lavrnja I, Stojkov D, Nedeljković N, Rakić L, Stojiljković M. Downregulation of glial scarring after brain injury: the effect of purine nucleoside analogue ribavirin. Ann NY Acad Sci. 2005,1048:296-310

Identification of Predictive Molecular Markers for Cancer Progression, Response to Therapy and Disease Outcome#41031

1. Stojsic J, Stankovic T, Stojkovic S, Milinkovic V, Dinic J, Milosevic Z, Milovanovic Z, Tanic N, Bankovic J. Prolonged survival after neoadjuvant chemotherapy related with specific molecular alterations in the patients with nonsmall-cell lung carcinoma. Exp Mol Pathol. 2014;98(1):27-32.
2. Dinić J, Novaković M, Podolski-Renić A, Stojković S, Mandić B, Tešević V, Vajs V, Isaković A, Pešić M. Antioxidative activity of diarylheptanoids from the bark of black alder (Alnus glutinosa) and their interaction with anticancer drugs. Planta Med. 2014;80(13):1088-96.
3. Milosevic Z, Pesic M, Stankovic T, Dinic J, Milovanovic Z, Stojsic J, Dzodic R, Tanic N, Bankovic J. Targeting RAS/MAPK/ERK and PI3K/AKT/mTOR signal transduction pathways to chemosensitize anaplastic thyroid carcinoma. Transl Res. 2014;164(5):411-23.
4. Mori M, Vignaroli G, Cau Y, Dinić J, Hill R, Rossi M, Colecchia D, Pešić M, Link W, Chiariello M, Ottmann C, Botta M. Discovery of 14-3-3 protein-protein interaction inhibitors that sensitize multidrug-resistant cancer cells to doxorubicin and the Akt inhibitor GSK690693. ChemMedChem. 2014;9(5):973-83.
5. Stankovic T, Milinkovic V, Bankovic J, Dinic J, Tanic N, Dramicanin T, Tanic N. Comparative analyses of individual and multiple alterations of p53, PTEN and p16 in non-small cell lung carcinoma, glioma and breast carcinoma samples. Biomed Pharmacother. 2014;68(5):521-6.
6. Liu Q, Manzano D, Tanić N, Pesic M, Bankovic J, Pateraki I, Ricard L, Ferrer A, de Vos R, van de Krol S, Bouwmeester H. Elucidation and in planta reconstitution of the parthenolide biosynthetic pathway. Metab Eng. 2014;23:145-53.
7. Novaković M, Pešić M, Trifunović S, Vučković I, Todorović N, Podolski-Renić A, Dinić J, Stojković S, Tešević V, Vajs V, Milosavljević S. Diarylheptanoids from the bark of black alder inhibit the growth of sensitive and multi-drug resistant non-small cell lung carcinoma cells. Phytochemistry. 2014;97:46-54.
8. Milinkovic V, Bankovic J, Rakic M, Stankovic T, Skender-Gazibara M, Ruzdijic S, Tanic N. Identification of novel genetic alterations in samples of malignant glioma patients. PLoS One. 2013;8(12):e82108.
9. Podolski-Renic A, Jadranin M, Stankovic T, Bankovic J, Stojkovic S, Chiourea M, Aljancic I, Vajs V, Tesevic V, Ruzdijic S, Gagos S, Tanic N, Pesic M. Molecular and cytogenetic changes in multi-drug resistant cancer cells and their influence on new compounds testing. Cancer Chem Pharmacol. 2013;72(3):683-97.
10. Banković J, Andrä J, Todorović N, Podolski-Renić A, Milošević Z, Miljković D, Krause J, Ruždijić S, Tanić N, Pešić M. The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: the unique way of multi-drug resistance modulation. Exp Cell Res. 2013;319(7):1013-27.
11. Dačević M, Isaković A, Podolski-Renić A, Isaković AM, Stanković T, Milošević Z, Rakić Lj, Ruždijić S, Pešić M. Purine nucleoside analog - sulfinosine modulates diverse mechanisms of cancer progression in multi-drug resistant cancer cell lines. PLoS One. 2013;8(1):e54044.
12. Jadranin M, Pešić M, Aljančić IS, Milosavljević SM, Todorović NM, Podolski-Renić A, Banković J, Tanić N, Marković I, Vajs VE, Tešević VV. Jatrophane diterpenoids from the latex of Euphorbia dendroides and their anti-P-glycoprotein activity in human multi-drug resistant cancer cell lines. Phytochemistry. 2013;86:208-17.

Five papers of IBISS researchers published in Drug Resistance Updates (IF = 18.5)

In 2020 and 2021, researchers from the Department of Neurobiology at the Institute for Biological Research "Siniša Stanković" - National Institute of Republic of Serbia (Laboratory for Molecular Neuro-Oncology) published 5 papers in the international journal Drug Resistance Updates (IF = 18.5). In Journal Citation Reports, Drug Resistance Updates is ranked among the top 3 journals in its science field (Pharmacology & Pharmacy).

Publications can be found at the following links:

Metal- and metalloid-based compounds to target and reverse cancer multidrug resistance

Repurposing old drugs to fight multidrug resistant cancers

What sustains the multidrug resistance phenotype beyond ABC efflux transporters? Looking beyond the tip of the iceberg

Advanced technological tools to study multidrug resistance in cancer

In vitro biomimetic models for glioblastoma-a promising tool for drug response studies

Call for symposia proposals for FRM2019

Don't miss your chance to influence the FENS Regional Meeting 2019 (FRM2019) by submitting a Proposal for Symposia.

FRM2019 will take place in Belgrade, Serbia, July 10-13, 2019 and will be organized by National Neuroscience Societies of Serbia, Romania and Turkey, under the auspices of Federation of European Neuroscience Societies (FENS). Deadline for symposia proposals is September 1st, 2018.

For more information, visit: www.fensfrm2019.rs/symposia/

COST Action CA17104 STRATAGEM

Dr. Milica Pešić, Dr. Ana Podolski-Renić, Dr. Tijana Stanković, Dr. Jelena Dinić, Dr. Sonja Stojković Burić and Miodrag Dragoj are participants in the COST Action CA17104 STRATAGEM (New diagnostic and therapeutic tools against multidrug resistant tumors) realized in the period from 2018 to 2022 and supported by COST (European Cooperation in Science and Technology). Co-proposer of the Action and Managment Committee (MC) member is Dr. Milica Pešić, while Dr. Ana Podolski-Renić performs the function of the MC substitute.

Lecture at the Global Young Academy Annual General meeting in Pattaya, Thailand

At the 8th International Conference of Young Scientists and Global Young Academy (GYA) Annual General meeting in Pattaya, Thailand, on May 10th 2018 Dr. Milica Pešić gave a lecture: "Detection and treatment of cancer drug resistance". Dr. Milica Pešić is the leader of GYA Working Groups Biodiversity for Survival via Biomedicine (Bio2Bio) and Women in Science (WiS).

Cover image of Histochemistry and Cell Biology 148(5)2017

Image from the article: Targeting autophagy to modulate cell survival: a comparative analysis in cancer, normal and embryonic cells, authored by: Aleksandra Divac Rankov, Mila Ljujić, Marija Petrić, Dragica Radojković, Milica Pešić, Jelena Dinić was selected for the cover image of Histochemistry and Cell Biology (Volume 148, Issue 5, November 2017).

Report from the 3rd Congress of the Serbian Association for Cancer Research

At the 3rd Congress of the Serbian Association for Cancer Research, held on 6-7 October 2017, our Institute was a co-organizer. Colleagues from the Department of Neurobiology and Department of Immunology participated in the organization and scientific section of the program on October 7, 2017. The panel discussion was conducted with international guest lecturers (Prof. Ignacio Ochoa Garrido from the University of Zaragoza, Spain, Prof. Engin Ulukaya from Istinye University in Istanbul, Turkey, Prof. Konstantinos Dimas from the University of Thessaly, Greece) and domestic participants from IBISS (Dr. Milica Pešić, Dr. Tijana Stanković, Dr. Sanja Mijatović, Dr. Jelena Dinić, and Dr. Ana Podolski-Renić).
In the photograph during a break between the lectures (from left to right) are: Prof. Engin Ulukaya, Prof. Konstantinos Dimas, Dr. Tijana Stanković, Prof. Ignacio Ochoa Garrido, Dr. Sonja Stojković Burić and Dr. Milica Pešić.

Lecture at the 4th Meeting of COST action CM1407 in Lisbon

At the 4th Meeting of COST action CM1407 in Lisbon, Dr. Milica Pešić gave a lecture: "Dual inhibitors in cancer tretment".
Dr. Milica Pešić is the leader of Working Group for cancer research within this COST action.

A recording of the lecture is available at the following link: https://drive.google.com/file/d/0B0z9_MnrOvGESDJCOE45UjA4UTQ/view?usp=drive_web

"Goran Ljubijankić" Foundation prize for the best doctoral dissertation in the field of molecular biology for 2015

Dr. Vesna Tešić won the prize from "Goran Ljubijankić" Foundation for the best doctoral dissertation in the field of molecular biology for 2015.

Neuroscience 2015 (304) Cover Winner

Figure 9 of the article entitled Impact of anesthetic regimen on the respiratory pattern, EEG microstructure and sleep in the rat model of cholinergic Parkinson disease neuropathology (Neuroscience, 2015, 304, 1-13) by Lazic K., Petrovic J., Ciric J., Kalauzi A., Saponjic J. was selected for the Cover Winner.

"Goran Ljubijankić" Foundation prize for the best doctoral dissertation in the field of biology for 2014

Dr. Zorica Milosevic won the prize from "Goran Ljubijankić" Foundation for the best doctoral dissertation in the field of biology for 2014.

Defence of doctoral dissertation

Zorica Milosevic, mentored by Dr. Jasna Banković, successfully defended her doctoral dissertation entitled "The Role of mTOR and MAPK signaling pathways in thyroid cancer resistance" at the Institute for Biological Research "Siniša Stanković", Belgrade.

“Academician Radoslav K. Andjus” Foundation Award 2013/2014

Dr Jelena Petrovic has been awarded by “Academician Radoslav K. Andjus” Foundation for the best publication in the field of physiology/biophysics in 2013/2014 entitled:

Lesion of the pedunculopontine tegmental nucleus in rat augments cortical activation and disturbs sleep/wake state transitions structure, Experimental Neurology, 2013, 247, 562-571, by Petrovic Ј, Ciric Ј, Lazic К, Kalauzi А, Saponjic Ј.

Dr. Ana Martins on training at the Department of Neurobiology

16.11.2014 - 12.04.2014 Dr. Ana Martins (Department of Medical Microbiology and Immunobiology, University of Szeged, Hungary) resided on training in methods for the detection of multi-drug resistance in cancer at the Department of Neurobiology under the supervision of Dr. Milica Pešić.

The best poster presentation

Dr. Ana Podolski-Renić won the first place in the competition for the best poster presentation entitled "Influence of MDR cancer phenotype on the efficacy of novel tubulin destabilizing agent DTA0100" at a meeting of COST CM1106 (http://www.stemchem.org) "2nd Workshop, 14 - 15 October 2014 Tenerife - Puerto de la Cruz, Spain."

New team member of the Ministry of Education, Science and Technological Development - Dr. Nikola Tanić

27.06.2014. Dr. Nikola Tanić, a Research Fellow at the Institute for Biological Research "Siniša Stanković", Belgrade, became a new team member of the Ministry of Education, Science and Technological Development. He holds the position of an assistant minister for the basic research and development of human resources in science.

Member of the "Global Academy youth" (Global Young Academy - GYA)

Dr. Milica Pešić has become one of the 200 members of the "Global Academy youth" (Global Young Academy - GYA), an organization that recognizes the best scientists from around the world at the beginning of their careers.

"Goran Ljubijankić" Foundation Award for the best doctoral dissertation 2013

Dr Ana Podolski-Renić was awarded by "Goran Ljubijankić" Foundation for the best doctoral dissertation (entitled "Establishment of resistant tumor cell lines as a model for testing new chemotherapeutics: molecular characterization of resistance caused by long term exposure to paclitaxel") in the field of biology for 2013.

Patent WO/2011/139172

Dr. Milica Pešić, Dr. Jasna Banković, Dr. Nikola Tanić and Dr. Sabera Ruždijić are co-inventors of the patent WO/2011/139172: DITERPENE COMPOUNDS WITH ANTINEOPLASTIC ACTIVITIES AND PHARMACEUTICAL COMPOSITIONS COMPRISING SAME

Diploma for participation in the final competition for "Best Technological Innovation in Serbia 2010"

Members of the team NEUROBIOS leaded by prof Mirjana Stojiljkovic won Diploma for participation in the final competition for "Best Technological Innovation in Serbia 2010", in the category REALIZED INNOVATIONS.

The second prize at the Competition for the "Best Technological Innovation in Serbia 2009"

Members of the team NEUROBIOS leaded by prof Mirjana Stojiljkovic won the second prize at the Competition for the "Best Technological Innovation in Serbia 2009", in the category POTENTIALS.

The second prize at the Competition for the "Best Technological Innovation in Serbia 2008"

Members of the team leaded by Dr. Sabere Ruzdijic won the second prize at the Competition for the "Best Technological Innovation in Serbia 2008", in the category POTENTIALS.

The first place at the International Festival of Innovations, Knowledge and Creativity – TESLA FEST

Part of the results of Laboratory for Neurochemistry won the first place at the International Festival of Innovations, Knowledge and Creativity – TESLA FEST and received Diploma and golden plaque of the Society of Inventors of Vojvodina.

1. Mujić A, Grdović N, Mujić I, Mihailović M, Živković J, Poznanović G, Vidaković M. Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells. Food Chem. 2011;125:841-9.

2. Mihailović M, Arambašić J, Uskoković A, Dinić S, Grdović N, Marković J, Poznanović G, Vidaković M. Alpha-lipoic acid preserves the structural and functional integrity of red blood cells by adjusting the redox disturbance and decreasing O-GlcNAc modifications of antioxidant enzymes and heat shock proteins in diabetic rats. Eur J Nutr. 2012;51:975-86.

3. Grdović N, Dinić S, Arambašić A, Mihailović M, Uskoković A, Marković J, Poznanović G, Vidović S, Zekovic Z, Mujić A, Mujić I. Vidaković M. The protective effect of a mix of Lactarius deterrimus and Castanea sativa extracts on streptozotocin-induced oxidative stress and pancreatic β-cell death. British J Nutr. 2012;108:1163-76.

4. Arambašić J, Mihailović M, Uskoković A, Dinić S, Grdović N, Marković J, Poznanović G, Bajec Đ, Vidaković M. Alpha-lipoic acid upregulates antioxidant enzyme gene expression and enzymatic activity in diabetic rat kidneys through an O-GlcNAc-dependent mechanism. Eur J Nutr. 2013;52(5):1461-73.

5. Doetsch M, Gluch A, Poznanović G, Bode J, Vidaković M. YY1-binding sites provide central switch functions in the PARP-1 gene expression network. PLoS One. 2012;7(8):e44125.

7. Dinić S, Uskoković A, Mihailović M, Grdović N, Arambašić J, Marković J, Poznanović G, Vidaković M. Ameliorating effects of antioxidative compounds from four plant extracts in experimental models of diabetes. J Serb Chem Soc. 2013;78(3):365-80.

8. Arambašić J, Mihailović M, Uskoković A, Dinić S, Grdović N, Marković J, Poznanović G, Bajec Đ, Vidaković M. Alpha-lipoic acid upregulates antioxidant enzyme gene expression and enzymatic activity in diabetic rat kidneys through an O-GlcNAc-dependent mechanism. Eur J Nutr. 2013;52(5):1461-73.

9. Marković J, Grdović N, Dinić S, Karan-Đurašević T, Uskoković A, Arambašić J, Mihailović M, Pavlović S, Poznanović G, Vidaković M. PARP-1 and YY1 are important novel regulators of CXCL12 gene transcription in rat pancreatic beta cells. PLoS One. 2013;8(3):e59679.

10. Dinić S, Arambašić J, Mihailović M, Uskoković A, Grdović N, Marković J, Karadžić B, Poznanović G, Vidaković M. O-GlcNAcylation of the key proteins in the kinase and redox signalling pathways is a novel mechanism of the beneficial effect of α-lipoic-acid in diabetic liver. Brit J Nutr. 2013;110(3):401-12.

11. Mihailović M, Arambašić J, Uskoković A, Dinić S, Grdović N, Marković J, Poznanović G, Ibrahim M, Vidaković M. β-glucan administration to diabetic rats reestablishes redox balance and stimulates cellular pro-survival mechanisms. J. Funct Foods. 2013;5(1):267-78.

12. Mihailović M, Arambašić J, Uskoković A, Dinić S, Grdović N, Marković J, Bauder J, Poznanović G, Vidaković M. β-Glucan administration to diabetic rats alleviates oxidative stress by lowering hyperglycaemia, decreasing non-enzymatic glycation and protein O-GlcNAcylation. J Funct Foods. 2013;5(3):1226-34.

13. Uskoković A, Mihailović M, Dinić S, Arambašić J, Grdović N, Marković J, Poznanović G, Vidaković M. Administration of a β-glucan-enriched extract activates beneficial hepatic antioxidant and anti-inflammatory mechanisms in streptozotocin-induced diabetic rats. J Funct Foods. 2013;5(4):1966-74.

14. Grdović N, Dinić S, Mihailović M, Uskoković A, Arambašić Jovanović J, Poznanović G, Wagner L, Vidaković M. CXC chemokine ligand 12 protects pancreatic β-cells from necrosis through Akt kinase-mediated modulation of poly(ADP-ribose) polymerase-1 activity. PLOS One. 2014;9(7):e101172.

15. Ivanović-Matić S, Mihailović M, Dinić S, Martinović V, Bogojević D, Grigorov I. Poznanović G. The absence of cardiomyopathy is accompanied by increased activities of CAT, MnSOD and GST in long-term diabetes in rats. J Physiol Sci. 2010;60(4):259-66.

16. Arambašić J, Poznanović G, Ivanović-Matić S, Bogojević D, Mihailović M, Uskoković A. Grigorov I. Association of the Glucocorticoid Receptor with STAT3, C/EBPß and the hormone responsive element within the rat haptoglobin gene promoter during the acute phase response. IUBMB Life. 2010;62(3):227-36.

17. Matić S, Stanić S, Bogojević D, Vidaković M, Grdović N, Arambašić J, Dinić S, Uskoković A, Poznanović G, Solujić S, Mladenović M, Marković J. Mihailović M. Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats. Can J Physiol Pharmacol. 2011;89(6):401-11.

18. Milošević VLj, Ajdžanović VZ, Bogojević DB, Medigović IM, Ivanović-Matić SK, Martinović VI, Grigorov I. The effect of chronic food restriction on immunopositive ACTH cells in peripubertal female rats. Gen Physiol Biophys. 2011;30(3):321-324.

19. Martinović V, Grigorov I, Bogojević D, Petrović A, Jovanović S, Ilić M, Ivanović-Matić S. Activation level of JNK and Akt/ERK signaling pathways determinates extent of DNA damage in the liver of diabetic rats. Cell Physiol Biochem. 2012;30(3):723-34.

20. Uskoković A, Dinić S, Mihailović M, Grdović N, Arambašić J, Vidaković M, Bogojević D, Ivanović-Matić S, Martinović V, Petrović M, Poznanović G, Grigorov I. STAT3/NF-κB interactions determine the level of haptoglobin expression in male rats exposed to dietary restriction and/or acute phase stimuli. Mol Biol Rep. 2012;39(1):167-76.

21. Arambašić J, Mihailović M, Bogojević D, Ivanović-Matić S, Uskoković A, Poznanović G, Grigorov I. Haptoglobin and the inflammatory and oxidative status in experimental diabetic rats: antioxidant role of haptoglobin. J Physiol Biochem. 2013;69(1):45-58.

22. Martinović V, Bogojević D, Jovanović S, Petrović A, Ivanović-Matić S, Zolotarevski L, Poznanović G, Grigorov I. Hepatoprotective effects of melatonin against pronecrotic cellular events in streptozotocin-induced diabetic rats. J Physiol Biochem. 2014;70(2):441-50.